A tidal wave of questions floats the need for more upstream automation.

The Making of mRNA

The milestone approval of a gene-edited therapeutic paves the way for gene-editing technologies.

Milestone Approval to Steady Stream, Gene Editing Revs Up

Both adherent and suspension cell culture approaches have their pros and cons, which must be considered for process development.

The Pros and Cons of Adherent Versus Suspension Cell Culture

Topic

Michael Mcilhatton, PhD, is a Senior Scientist, process development lab, at Andelyn Biosciences.

is a Senior Scientist, process development lab, at Andelyn Biosciences.

Sarah Bergman is a scientist, process development lab, at Andelyn Biosciences.

Brittany Hurley is a scientist, process development lab, at Andelyn Biosciences.

scientist, process development, at Andelyn Biosciences.

Andrew Moreo oversees process development, preclinical plasmid production, and viral vector production at Andelyn Biosciences.

Associate Director of Process Development at Andelyn Biosciences.

Articles

This commentary summarizes the derivation of clonal HEK293 suspension cell lines, selection of clones for rAAV production, and design of experiments-based optimization strategies for characterization of one clonal isolate for high yield rAAV manufacture. Data shown provides proof of concept for the success of this strategy, its applicability for manufacturing different rAAV serotypes and lays the foundation of further clonal cell line characterization for scale up of production.

Strategy for Derivation and Optimization of a Clonal HEK293 Suspension Cell Line for High Yield AAV Production

Chris Spivey is the editorial director of 

Videos

Global product manager at Cytiva, Eric Corti, discusses the challenges with designing a new single-use mixing system that ensures leak-free fluid handling operations at INTERPHEX 2024.

Exploring Innovative Engineering Design to Address Upstream Bioprocessing Complications (INTERPHEX 2024)

This investment will bolster the company’s large-scale cell culture CDMO business at its planned Fujifilm Diosynth Biotechnologies manufacturing facility in Holly Springs, North Carolina.

Fujifilm Invests $1.2 Billion in Cell Culture Bio-Manufacturing Facility Expansion

Feliza Mirasol is the science editor for 

Despite a growing trend toward single-use bioreactors, stainless-steel retains its relevance for certain products.

Evaluating Uses for Both Single-Use and Stainless-Steel Bioreactors

Cameron Graham is senior process engineer, Biologics at BIOVECTRA.

Gregor Awang is specialist, Biologics Development and Innovation at BIOVECTRA.

Challenges in fermentation can be addressed through equipment changes, facility design, and process development.

High-Growth Microbial Fermentation for the Manufacture of Biologics

Abzena has launched its AbZelect platforms, designed for improving cell line development.

Abzena Launches New Cell Line Development Platforms

William Tran is a senior scientist at Biologics Process R&D, Merck and Co. (MSD outside of the United States and Canada).

Jack Bowers is a senior principal scientist at Biologics Process R&D, Merck and Co. (MSD outside of the United States and Canada).

T. Craig Seamans is a senior principal scientist at Biologics Process R&D, Merck and Co. (MSD outside of the United States and Canada).

Helena Öhrvik is senior product manager at In Silico Process Development, Cytiva.

Andreas Castan is strategic technology leader at In Silico Process Development, Cytiva.

The authors give an overview of scaling approaches and present good scaling practices for the biopharma industry.

Right the First Time: Bioreactor Scale and Design Translation

Ann Rossi Bilodeau, PhD, is senior bioprocess applications scientist at Corning Life Sciences.

Upstream Processing

The Making of mRNA

Milestone Approval to Steady Stream, Gene Editing Revs Up

The Pros and Cons of Adherent Versus Suspension Cell Culture