Storing an Ion-Exchange Chromatography Gel in Dilute Alkali During Recycling Improves Cleaning

Publication
Article
BioPharm InternationalBioPharm International-04-01-2002
Volume 15
Issue 4

by Jane Turton, Central Public Health Laboratory and Zain Moola This case study shows that storing an ion-exchange chromatography gel in dilute alkalai, followed by removal of the solubized material, enhances gel cleaning. More material is removed from the gel during column recycling, and carry over of material from one cycle to the next is substantially reduced.

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