Rapid Low-level Identification and Quantitation of Host Cell Proteins

Register free: http://www.biopharminternational.com/bp_w/proteins

Event Overview:

Process impurities such as host cell proteins (HCPs) can threaten product efficacy and stability and, therefore, must be monitored and controlled in drug products. 

The traditional enzyme-linked immunosorbent assay (ELISA) method lacks the specificity and coverage to identify and quantify individual HCPs. Liquid chromatography (LC)–mass spectrometry (MS) analysis overcomes that these limitations; however, the coelution of low-abundance HCP peptides with the highly abundant peptides from the drug product demands better separation of the peptides and broad dynamic range of the LC/MS system.

In this webcast, experts will review an HCP analysis workflow for automated sample preparation; an LC–MS/MS analysis using a new acquisition method shown to improve protein identification; and data analysis software to confirm identification of HCPs.

Key Learning Objectives:

How to optimize each stage of a host cell protein workflow including sample prep, separation, detection, and data processing

How a new acquisition method can identify lower-level peptides

How to perform targeted quantitation of HCPs

Speakers: Steve Madden, Software Product Manager, Agilent Technologies

Linfeng Wu, PhD, Application Scientist, Agilent Technologies

Time and Date: Europe: Thursday, October 10, 2019 at 2pm BST | 3pm CEST

North America: Thursday, October 10, 2019 2pm EDT | 1pm CDT | 11am PDT

On demand available after final airing until Oct. 10, 2020

Sponsor: Agilent Technologies

Register free: http://www.biopharminternational.com/bp_w/proteins