Native MS for Aggregate and Charge Variant Analysis of Biotherapeutics
Aggregate analysis and charge variant analysis using a native MS approach allows rapid analysis of biotherapeutic critical quality attributes. Learn about these techniques from experts at Phenomenex and SCIEX. Live: Tuesday, Aug. 20, 2019 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST On demand available after airing until Aug. 20, 2020 Register free
Register free: http://www.chromatographyonline.com/lcgc_w/charge_variant
Event Overview:
Size exclusion chromatography (SEC) and ion exchange chromatography (IEX) are classic techniques employed in the characterization of biotherapeutics under non-denaturing conditions. Standard mobile phase conditions for these techniques are high in salt concentration, rendering them incompatible with mass spectrometry (MS) detection. Native mass spectrometry (MS) represents a cutting-edge approach to employ MS-compatible mobile phases for non-denaturing SEC and IEX analysis. This webcast will discuss aggregate and charge variant analysis using native mode MS.
Key Learning Objectives
- Understand fundamentals in SEC and IEX for biotherapeutic characterization
- Effect of mobile phase conditions and chromatographic parameters in aggregate analysis by native SEC
- Determination of acidic and basic variants of monoclonal antibodies using native weak cation exchange chromatography (WCX)
Speaker: Dr. Chris Nortcliffe, Applications Specialist, SCIEX
Carl Sanchez, Senior Research Scientist, Phenomenex
Time and Date: Tuesday, Aug. 20, 2019 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST
On demand available after airing until Aug. 20, 2020
Sponsor: Phenomenex and Sciex
Register free: http://www.chromatographyonline.com/lcgc_w/charge_variant
