Break Through the Boundaries of Purity Analysis of Long Guiding RNAs for New CRISPR Gene Editing Systems

Take control of the purity of your prime editing guide RNAs and overcome secondary structure formation with a solution that offers the highest levels of resolution separation and repeatability.

Register Free: https://www.biopharminternational.com/bp_w/gene-editing

Event Overview:

Prime editing (PE) is a next-generation gene editing technology that utilizes a Cas9 protein fused to a prime editing guide ribonucleic acid (pegRNA) to achieve high editing efficiency and precision. However, the length requirement of pegRNAs at 120–250 nucleotides (nt) and their high level of secondary structure formation present analytical challenges for the purity analysis of chemically synthesized pegRNAs during development and quality control (QC).

In this webinar, you will learn how to mitigate technical challenges by disrupting the hydrogen bonds in pegRNA molecules and maintaining their denatured state during analysis using a method based on capillary gel electrophoresis (CGE). This method offers high resolution and provides purity quantification of these large, synthetic oligonucleotides.

Key Learning Objectives
Learn how to:

• Overcome high amounts of secondary structures of large pegRNAs for next-generation CRISPR/Cas9 gene editing approaches.
• Determine purity of synthetic pegRNA with high-resolution separation of the full-length product from its impurities.
• Achieve a high level of repeatability with a relative standard deviation (RSD) of <3%.

Who Should Attend
Scientists, Sr. Scientists, Lab Managers, Lab Directors

Speakers:

Ashley Jacobi
Senior Manager of Applications Development, Genomics Medicine Integrated DNA Technologies

Tingting Li
Manager, Cell and Gene Therapy Applications
SCIEX

Register Free: https://www.biopharminternational.com/bp_w/gene-editing