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The pharmaceutical industry’s pipeline of new therapeutics has experienced a major shift from small to large molecules in recent years. As biopharmaceutical development expands, so too does the need for analytical techniques that address the key difficulties in this area. In Advances in Biopharmaceutical Characterization: Charge Variants and Aggregates, experts discuss overcoming the challenges of developing mAb formulations using novel pH gradient methods for ion exchange chromatography that provide fast and reproducible mAb charge variant profiling.