Brittany Hurley

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Brittany Hurley is a scientist, process development lab, at Andelyn Biosciences.

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Strategy for Derivation and Optimization of a Clonal HEK293 Suspension Cell Line for High Yield AAV Production

Michael Mcilhatton;John Ketz;Sarah Bergman;Brittany Hurley;Byron Carper;Andrew Moreo;Samir Acharya
May 6th 2024

This commentary summarizes the derivation of clonal HEK293 suspension cell lines, selection of clones for rAAV production, and design of experiments-based optimization strategies for characterization of one clonal isolate for high yield rAAV manufacture. Data shown provides proof of concept for the success of this strategy, its applicability for manufacturing different rAAV serotypes and lays the foundation of further clonal cell line characterization for scale up of production.

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