Whitepapers

In this study, HEK293F cells grown in scale-X bioreactor produce >3x higher AAV-2 vector, with 25-52% lower cost than the reference process.

This study aims to evaluate harvest strategies and metabolite-based cell density estimation methods for Lentivirus production with HEK293T cells.

BridgeBio Pharma, Inc., and REGENXBIO Inc. take a novel approach to AAV capsid titer quantification by using Octet® AAVX Biosensors to generate viral titer data.

Discover how the Ramina Process Analyzer integrates with the 500L HyPerforma DynaDrive bioreactor to perform accurate in-line measurements and prediction models.

This application note examines the suitability of BioBLU® 0.3c Single-Use Bioreactors and the DASbox Mini Bioreactor System in the long-term expansion of CD4+ T cells.

This application note demonstrates the successful transfer, optimization and scale-up of the Rubella vaccine manufacturing process from static flasks to scale-X™ bioreactors.

This application note demonstrates the successful transfer, optimization and scale-up of the Rubella vaccine manufacturing process from static flasks to scale-X™ bioreactors.

The combination of the PentaMice® platform and single B cell screening with the Beacon® system increases speed to market for monoclonal antibody antibody therapeutics.

This application note explores the feasibility of utilizing the scale-X carbo bioreactor as a solution for seed train intensification.

Using the BioAccord LC-MS System and an Ambr® 15, we developed an analytical method for rapid mAb glycoform confirmations and mAb purity assessments. This unique method is high throughput capable and has the potential to be a fully automated platform for mid-process intraday monitoring of CQAs.

Modulating osmolality in the early stages of AAV1 upstream manufacturing, can result in improved outcomes such as overall yield, quality, reduced manufacturing costs and improved efficiencies.

In this work we demonstrate the effectiveness of Design of Experiment methodology for upstream process development through the execution of transient gene expression optimization and oxygen mass transfer characterization studies.

In this paper, we present an alternative targeted metabolite profiling approach using high resolution accurate mass (HRAM) mass spectrometry with full scan analysis.

Wastewater monitoring has been widely adopted by scientists to effectively detect and monitor infectious pathogens. Methods of detection and surveillance for SARS-CoV-2 are outlined in this paper and can be adapted for other pathogens.

Roche Pharma Research and Early Development lab automation specialist, Pawel Linke, discusses their approach to doubling the number of projects in process development without significantly increasing headcount.

Read a case study about resin screening, process development, and scale-up purification of a retrovirus-like particle which was scalable, fast, and low-cost during the clinical-grade manufacture of retrovirus vectors.   

Used by leading pharma companies and CDMOs, the Beacon system offers an integrated end-to-end automated workflow for mammalian cell line development. Clones can be selectively enriched for unique phenotypes and multiple assays can be used to rapidly measure productivity of different antibody molecules.

The proportional–integral–derivative (PID) control mechanism is used for a variety of processes, including dissolved oxygen (DO) control. This protocol introduces a method developed to optimize the PI values on Eppendorf bioprocess controllers.

Traditional sequential chromatography usually requires user manipulation which may hinder reproducibility in protein purification runs. Multidimensional chromatography improves the process without sacrificing efficacy. This study compares both purification workflows demonstrating equivalent purity.

• Using stirred-tank bioreactors for stem cell culture provides advantages. • Factors to consider include OTR, agitation speed, and impeller blades. • An eight-blade impeller can address challenges associated with scale-up.

The Synergy Tube is a BCT specifically designed to optimize nucleic acid recovery and cancer tumor cells (CTC) from the point of blood draw. The Synergy Tube and proprietary stabilizer dramatically improve the quantity and quality of these components for downstream genetic diagnostics.