Capture of a scFv from E.coli
Method for capture of scFV and post-capture-washing condition with chaotropic agents is described. The post-capture washing condition significantly improved HCP removal to increase scFV purity.
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Glycan analysis provides key information on critical quality attributes that could affect stability, safety and efficacy of a protein therapeutic. Specific needs for understanding the glycosylation profiles change throughout the drug development process, but the requirement for high-resolution glycan information remains the same and is essential to help ensure product quality.
