The past few years have been filled with seminars discussing viruses, with COVID-19 stealing most of the headlines. Not all viruses are created equal; some are actually beneficial for human health and biotherapeutics delivery. Adeno-associated virus (also referred to as AAV) vectors fall into this category. AAVs are “hot” right now, as they are currently one of the most promising systems for human gene therapy. There are many types of AAVs and purification and analysis of these types of viruses can present challenges. In this dedicated dialogue, we will discuss the most efficient workflow for AAV purification and analysis.