Principles and Practical Steps to Develop Robust Reversed Phase Separations of Peptides

Reverse phase chromatography is the predominant technique used for the analyses and or purification of chemically synthesize peptides or analysis of peptides generated by the enzymatic digestion of proteins (i.e., peptide mapping).

This webinar will review some of the basic principles using reverse phase chromatography for peptide separations. It will conclude with discussion of a simplified step-by-step approach to develop a method that can be applied to chemically synthesized peptides or protein digest separations.

Register Free: https://www.chromatographyonline.com/lcgc_w/peptides

Event Overview:

Reverse phase chromatography is the predominant technique used for the analyses and or purification of chemically synthesize peptides or analysis of peptides generated by the enzymatic digestion of proteins (i.e., peptide mapping).

This webinar will review some of the basic principles using reverse phase chromatography for peptide separations. It will conclude with discussion of a simplified step-by-step approach to develop a method that can be applied to chemically synthesized peptides or protein digest separations.

Key Learning Objectives:

• How column chemistry and RP mobile phase affects peptide separation selectivity
• Review the factors that synergistically work to obtain desired resolution of peptides contained in what could be a complex mixture
• What to consider when selecting an appropriate reversed phase column for peptide separations

Featured Speaker:

Bill Warren
Principal Consultant Product
Manager
Waters Corporation

In 1986, Bill Warren joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids. He currently is a Principal Consulting Bioseparations Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.

Register Free: https://www.chromatographyonline.com/lcgc_w/peptides