Achieving High Productivities through Bioreactor Optimization at Lower than Peak Cell Densities in a Perfusion Process

Explore methods that ensure your perfusion process maximizes mAb production. Join us as we reveal the latest techniques for achieving high productivity in a continuous steady-state perfusion culture with BalanCD CHO Perfusion. Discover how optimizing bioreactor parameters and using the right cell culture medium can put you at the forefront of bioprocessing efficiency and innovation

Register Free: https://www.biopharminternational.com/bp_w/bioreactor-optimization

Event Overview:

he demand for producing therapeutic recombinant proteins is generating a renewed interest in perfusion cell culture technologies, leading to the development of cell culture media that can support high cell densities (HCDs). However, HCD in bioreactors can generate challenges due to the requirement to maintain highly productive cells over long periods of time. Implementing a perfusion process that uses a production bioreactor is costly, requiring small scale models to optimize the process. BalanCD CHO Perfusion and Perfusion Media Survey Panel were evaluated with perfusion mimic models and a table-top perfusion capable bioreactor, featuring an alternating tangential flow filtration unit in both an N-1 perfusion process and a recombinant antibody production process. In this webcast, we demonstrate the optimization of a perfusion process in a table-top perfusion capable bioreactor, scaling-up from small scale perfusion mimic models. We assayed peak cell densities in an N-1 perfusion process demonstrating how productivity is related to various metabolites, glucose, and aeration during a continuous steady-state culture. Our results demonstrate success of achieving high productivities in a continuous steady-state perfusion culture through the optimization of bioreactor parameters, while utilizing an appropriate cell culture medium without achieving peak cell densities.

Key Learning Objectives:

• Learn how to use perfusion mimic models to assess a perfusion medium.
• Understand how to scale from a micro bioreactor to a table-top perfusion capable bioreactor.
• Master the optimization of the perfusion process to achieve increased productivity.

Who Should Attend:

• Process Development Scientists: Early Clinical
• Manufacturing Managers/Directors: Early Clinical
• Process Development Scientists: Biotech
• Manufacturing Managers/Directors: Biotech
• C-Suite/Senior Management: Pharma
• C-Suite/Senior Management: Early Clinical

Speaker:

Luis Rodriguez, PhD
Bioproduction R&D Manager
FUJIFILM

Luis Rodriguez, PhD, is a bioproduction research and development manager, at FUJIFILM Irvine Scientific. In this role, he heads the life science bioprocessing team to lead the development of new media, advance process improvements, and provide technical support for the production of biotherapeutics. Dr. Rodriguez received his BS in cell and molecular biology from California State University-Northridge and his PhD in biochemistry, molecular, and cellular biology from Cornell University.


Register Free: https://www.biopharminternational.com/bp_w/bioreactor-optimization