Sartorius

Live-cell imaging enables acquisition of phase contrast images and provides an ideal platform to study multi-faceted biological paradigms in drug discovery. This is vital to our understanding of human diseases and treatment strategies. The movement of these models towards increasingly complex physiologically relevant ones, including patient-derived cells and induced pluripotent stem cells (iPSCs), has concurrently driven the need for label-free methods that are non-perturbing to deliver deeper biological insights.

The response of tumor cells, such as glioblastomas, to cytotoxic chemotherapeutic compounds is a complex and dynamic process that is crucial to understand in neuro-oncology research. Using pre-trained neural networks to accurately segment individual cells and classify them as live or dead is a powerful, robust approach for assessing cytotoxicity in glial cell types.

Examine the dynamic changes in cell morphology which occur when cells undergo ferroptosis and investigate label-free and fluorescence-based methods of kinetic quantification.

Phenotypic screening is a vital tool in drug discovery, enabling scientists to rapidly identify potential drugs early in the discovery pipeline. Kinetic information gained by repeated image acquisition within a physiologically relevant environment provides insight into the dynamic changes which are often overlooked using end-point analysis alone.

Webinar Date/Time: Tue, Jun 11, 2024 10:00 AM EDT

The evolution of regulatory guidelines for biosimilars alongside improvements in knowledge and understanding provide a platform for growth in the industry. Technology to support their rapid growth must simplify and streamline processes during drug development.

Webinar Date/Time: Wed, Jul 19, 2023 11:00 AM EDT

BridgeBio Pharma, Inc., and REGENXBIO Inc. take a novel approach to AAV capsid titer quantification by using Octet® AAVX Biosensors to generate viral titer data.

The rapid development of gene therapies and high demand for therapeutic proteins pose considerable challenges for biomanufacturing. Complex production processes, product changeover, managing supply chains, and staying ahead of the curve, to name just a few. To accelerate commercialization of biotherapeutics, organizations need to optimize processes by integrating advanced analytical tools that maximize quality, safety, and efficacy. The solution could be a comprehensive approach for screening and characterizing molecular interactions such as protein-protein or protein-drug interactions. It enables a huge variety of applications performed at various stages of biologics development — from early selection to validation to manufacturing. Based on Bio-Layer Interferometry (BLI), it enables real-time, label-free analysis for the determination of kinetics, affinity, and antibody/protein quantitation. Download this eBook to learn how the Octet® BLI platform can streamline workflows, reduce time-to-results, and costs saving on broad range of analytical applications in bioprocessing.

Webinar Date/Time: Wednesday, March 29th 2023 7am PDT | 10am EST | 4pm CEST

Adeno-associated virus (AAV) is a widely used viral vector for the delivery of genetic material in gene therapy. Vector development and production at an industrial scale requires an efficient method for accurate quantification of viral capsids. Unfortunately, traditional techniques such as ELISA, HPLC, and ddPCR are time consuming and labor-intensive. This podcast introduces a rapid, label-free method for the direct capture and quantitation of various AAV serotypes using the Octet® Bio-Layer Interferometry (BLI) platform with Octet® AAVX Biosensors. These biosensors deliver results in as little as 15 minutes and can be used to quantitate AAV capsids in both crude and purified samples.

A completely hands-off, walk-away HCP assay analyzing 96 samples can be set up to run automatically on an Octet® RH96 instrument with results obtained in one hour.

To accelerate commercialization of biotherapeutics, organizations need to optimize processes by integrating advanced analytical tools that maximize quality, safety, and efficacy. Octet® BLI systems enable real-time, label-free analysis for the determination of kinetics, affinity, and antibody/protein quantitation. Based on Bio-Layer Interferometry (BLI), the systems enable an enviable variety of applications throughout biologics development, from early selection to validation and manufacturing. Read on to learn about the implementation in the production of biotherapeutics for titer, host cell protein analysis, residual protein detection, sialic acid content detection, potency assay development, Fab activity assay development, and more.

A more streamlined technique with reduced hands-on time using the Octet® platform. For customers who require a sensitive and robust assay for measuring small amounts of leached Protein A in antibody or Fc-fusion protein samples.

Replace ELISA and HPLC with Octet® systems for quantitation of antibodies and recombinant therapeutic proteins. Accurate and fast assays are also easy to develop and transfer to QC and manufacturing. In this whitepaper you will learn about analyzing a full plate (96-samples) of IgG titer in as little as two minutes, assay directly in crude and unpurified samples, and automation for extending un-attended run times and high throughput analysis.

In this White Paper, Sartorius outlines the challenges of scaling a bioprocess and presents solutions to maintain control over critical quality attributes.

Replace ELISA and HPLC with Octet® systems for quantitation of antibodies and recombinant therapeutic proteins. Accurate and fast assays are also easy to develop and transfer to QC and manufacturing.

Here, Sartorius provides an overview of cell line development and the associated hurdles. We will then discuss potential solutions that form part of an effective risk-management strategy and improve productivity.

Comparison of HPLC and Octet® system to determine MAb concentration in a complex feedstock using mAbs breakthrough as the analyte while monitoring method accuracy, precision, dynamic range, LoQ and cost per sample.

Pharmacopoeias are collections of recognized and binding quality regulations that specify, among other things, test requirements and methods for the analysis of drugs. Both the European Pharmacopoeia (Ph. Eur.) and the United States Pharmacopeia (USP) contain specific chapters that deal with the use of laboratory balances. The chapters emphasize the criticality of weighing samples accurately in pharmaceutical analytical processes. The aim of this white paper is to present the testing requirements for laboratory balances as described in the relevant chapters of the pharmacopoeias and the associated certificates offered by Sartorius Service.

The purity of the solvents used, primarily that of water, is a decisive criterion for interference-free and reproducible analysis by liquid chromatography and for the sensitivity of this method, especially for applications in trace analysis. In a study, different sources of ultrapure water used as eluents were compared in high-performance liquid chromatography with diode-array detectors (HPLC-DAD) and mass spectrometry (MS) systems in various experiments

The Cubis® II balance series was designed for customizable modularity, which means the user can choose from many hardware and software options for thousands of different configurations. Choose from among 45 weighing modules, seven draft shields, two display and control units, and five software packages, including more than 60 software Apps.

In many laboratories, space is at a premium. Especially when centralized laboratory water systems are required to dispense pure and ultrapure water for your applications. To maximize your lab space capabilities, you shouldn’t have to adapt your water system to the laboratory. The system should adapt to your needs. In addition to being economical in terms of its footprint, flexibility in dispensing water is essential. Users need full control over quality parameters at the point of use and the ability to fill different sized containers at the same time across the lab. Sartorius will discuss an innovative and flexible lab water solution that addresses all of these requirements.

Water is a major component of all cell culture media and is, therefore, needed to prepare media, buffers, and additives, as well as to serve many ancillary functions, such as heating, cooling, cleaning and rinsing. Thus, water quality plays an important role in the outcome of cell culture experiments.

HPLC is an analytical procedure for separation, identification and quantification of substances using liquid chromatography. The beginnings of HPLC – High Pressure Liquid Chromatography – go back to the 60’s. Thanks to improved column materials and equipment, it has come to be known as High Performance Liquid Chromatography since the end of the 70’s.

In this Q&A, BioPharm International spoke with Sebastian Weber, product manager for lab weighing at Sartorius, about how to achieve data integrity, minimize risk and the potential for FDA violations, and how digital workflows can be supported by technology like the Cubis II lab balance.

Whitepaper on the importance of a robust biopharmaceutical cold chain. Explore the logistical challenges involved in cold chain transport. Read about critical drivers for solution selection by biologic, developmental phase, and operations.

Wednesday, July 14, 2021 at 11am EDT| 8am PDT| 4pm BST| 5pm CEST

Here, Sartorius provides an overview of cell line development and the associated hurdles. We will then discuss potential solutions that form part of an effective risk-management strategy and improve productivity.