Process Design of a Two-Step mAb Purification Using a Multi-Column Continuous Chromatography Platform

Register Free: https://www.biopharminternational.com/bp/chromatography

Event Overview:

Biotherapeutics, such as monoclonal antibodies (mAbs) and their modifications, have typically relied on multi-step processes for optimal removal of impurities such as host cell proteins (HCPs), DNA, adventitious viruses, and antibody aggregates. However, additional purification steps increase downstream expenses significantly, including costs of supplementary resin, hardware, buffers, etc. Thus, it is imperative to design and test effective purification procedures for production of high-quality biotherapeutics, but with reasonable production costs, time, and manufacturing space requirements.

• Multi-column continuous chromatography (MCC) is a powerful process intensification tool to unlock increased productivity and to address these purification concerns.
• However, the design of such processes can seem daunting, especially for multiple process steps. This event describes a process outline to design and obtain the benefits of MCC technology.

Key Learning Objectives:

• Design a powerful and robust, 2-step, mAb purification process directly in a MCC format.
• Optimize both intensified capture and polishing processes and complete an MCC proof of concept with one system and limited material.

Who Should Attend:

• Chromatographers, method developers, and process engineers who are involved with the development and tech transfer of MCC processes.
• Researchers evaluating geothermal brines.

Speakers

Jukka Kervinen, Ph.D.
Manager, Applications Development
Tosoh Bioscience

Jukka’s industry background is in the development of protein purification processes for monoclonal antibodies, proteolytic enzymes, and vaccine candidates. He currently directs chromatographic method testing for monoclonal antibody, oligonucleotide, and viral vector purification processes and their U/HPLC analysis at Tosoh Bioscience.

Jaclyn Mabry
Applications Scientist – MCC Instruments
Tosoh Bioscience

Jaclyn’s industry background is in the testing of protein purification platforms for monoclonal antibodies by converting known batch purification strategies for use with multi-column continuous chromatography instrumentation. Her current responsibilities at Tosoh Bioscience consist of testing novel uses of their multi-column chromatography instrumentation for the purification development of biopharmaceuticals.

Register Free: https://www.biopharminternational.com/bp/chromatography