Novel Oligonucleotide Sample Clean-up and Method Optimizations

Register Free: https://www.chromatographyonline.com/lcgc/novel

Event Overview:

Oligonucleotides represent a growing therapeutic modality. In particular, sample preparation and extraction of oligos from various biological matrices can be quite complex. However, depending on the type of biological matrix, the standard sample preparation approaches may not have the sample recovery nor sensitivity necessary for the method. Further, depending on the sample volume, some of the traditional methods may not yield acceptable results.

In this webinar, we will give an overview of sample preparation strategies for synthetic oligonucleotides, including how to adjust experimental design depending on the oligo and sample matrix. The presentation will also go into depth about the critical parameters to adjust to ensure effective sample preparation and cleanup methods are implemented. Additionally, method optimization for small volume sample extraction and clean-up solutions for oligonucleotides will be explored to further optimize challenging methods.


Key Learning Objectives:

• Introduction to oligonucleotides and the chromatographic challenges that arise when working with them
• Optimization of solid-phase extraction (SPE) methods for clean-up of different types of oligonucleotides
• Recommendations for improving SPE methods for small sample volumes
• Recommendations for the chromatography for diverse oligos

Who Should Attend:

• Biopharmaceutical chemists new to working with oligonucleotides
• Biopharmaceutical scientists needing to improve method sensitivity
• Anyone new to oligonucleotide characterization by LC-MS

Speaker

Mihir Thakar
Global Product Manager, Sample Preparation Biopharma
Phenomenex

Register Free: https://www.chromatographyonline.com/lcgc/novel