Featured Resources for Oligonucleotides Separations
Fundamentals of Oligonucleotide Separation by HPLC
From siRNA to mRNA to DNA, separating full-length product from impurities can be challenging. Depending on the nature of the oligonucleotide you are interested in, you will be able to make informed choices about what approach to use to characterize or purify your molecule.
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Evaluation of New High pH Stable, Superficially-porous Particle Columns for the Reversed-phase Separation of Oligonucleotides
In this work, we evaluate the uses of new high pH stable, superficially-porous particles for oligo separations for fast and high resolution analysis, and compare them with totally porous particles.
High Performance DNA Oligonucleotide Purification Using Agilent TOP-DNA
Trityl-on purification enhances the selectivity between the full-length sequence and the truncated failures. Solid phase extraction (SPE) tubes provide a low-cost method for this type of purification. Read about how Agilent TOP-DNA cartridges make it easy to quickly obtain outstanding yields of high-purity synthetic DNA oligonucleotides.
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Fast and High-Resolution Reversed-Phase Separation of Synthetic Oligonucleotides
In this application note, we demonstrate fast and high-resolution separation and identification of a number of de-protected (removal of the dimethoxytrityl group (DMT)) DNA and RNA oligonucleotides, using Agilent AdvanceBio Oligonucleotide columns.
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High Resolution Separations of Oligonucleotides Using PL-SAX Strong Anion-Exchange HPLC Columns
In this application note, you can learn how to obtain fast and high-resolution separations and identifications of de-protected DNA and RNA oligonucleotides to ensure High-purity products.
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Direct Analysis of In-Process Oligonucleotides Without Manual Purification
Learn about direct 2D-LC analysis of oligonucleotides in solution with high salt content and online desalting performed in the first dimension (1D), followed by IP-RPLC analysis in the second dimension (2D) in this application note.
Are Current Dissolved CO2 Measurement Technologies Good Enough?
November 15th 2024Download Part 2 of the DCO₂ White Paper Series and learn the shortcomings of current measurement technologies. As demonstrated in Part 1, Dissolved CO₂ is a Critical Process Parameter (CPP) in biopharma production processes. However, the sensors currently used to measure and monitor this CPP each have significant drawbacks.
Biopharma Downstream Critical Process Parameters (Nov 2024)
November 15th 2024Download our comprehensive white paper and gain valuable insights into the critical process parameters and advance sensor technologies that can revolutionize your Biopharma Downstream Process (DSP). Discover strategies and techniques to optimize efficiency, improve product quality, and ensure the safety and efficacy of your biopharmaceutical products.
White Paper: Measurement Challenges with Optical Dissolved Oxygen Sensors
November 15th 2024Are you missing sources of measurement error? Measurement of dissolved oxygen (DO) has long been known as a critical control parameter for optimizing cell growth in bioprocesses. Much has been written about different strategies for proper DO control. However, little information has been publicly available regarding optimization of the actual measurement accuracy and reducing common sources of process-related error. Our White Paper aims to divulge these details for the first time.
Cell Density Applications eBook
November 15th 2024Collect More Actionable Data and Optimize Yield with Real-Time Process Adjustments. Learn more how Viable Cell Density Monitoring works for your process in Hamilton’s eBook that features must-see examples of real applications using Hamilton viable cell density monitoring (Incyte) and total cell density monitoring (Dencytee).
