Featured Resources for Oligonucleotides Separations
Fundamentals of Oligonucleotide Separation by HPLC
From siRNA to mRNA to DNA, separating full-length product from impurities can be challenging. Depending on the nature of the oligonucleotide you are interested in, you will be able to make informed choices about what approach to use to characterize or purify your molecule.
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Evaluation of New High pH Stable, Superficially-porous Particle Columns for the Reversed-phase Separation of Oligonucleotides
In this work, we evaluate the uses of new high pH stable, superficially-porous particles for oligo separations for fast and high resolution analysis, and compare them with totally porous particles.
High Performance DNA Oligonucleotide Purification Using Agilent TOP-DNA
Trityl-on purification enhances the selectivity between the full-length sequence and the truncated failures. Solid phase extraction (SPE) tubes provide a low-cost method for this type of purification. Read about how Agilent TOP-DNA cartridges make it easy to quickly obtain outstanding yields of high-purity synthetic DNA oligonucleotides.
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Fast and High-Resolution Reversed-Phase Separation of Synthetic Oligonucleotides
In this application note, we demonstrate fast and high-resolution separation and identification of a number of de-protected (removal of the dimethoxytrityl group (DMT)) DNA and RNA oligonucleotides, using Agilent AdvanceBio Oligonucleotide columns.
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High Resolution Separations of Oligonucleotides Using PL-SAX Strong Anion-Exchange HPLC Columns
In this application note, you can learn how to obtain fast and high-resolution separations and identifications of de-protected DNA and RNA oligonucleotides to ensure High-purity products.
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Direct Analysis of In-Process Oligonucleotides Without Manual Purification
Learn about direct 2D-LC analysis of oligonucleotides in solution with high salt content and online desalting performed in the first dimension (1D), followed by IP-RPLC analysis in the second dimension (2D) in this application note.
Biophysical and Aggregate Characterization for the Development of Biologics
June 10th 2025This eBook explores how advanced particle analysis technologies are revolutionizing biologic drug development. It highlights the importance of accurately identifying and characterizing subvisible particles to ensure drug safety, stability, and regulatory compliance. Techniques like Backgrounded Membrane Imaging (BMI) and Fluorescence Membrane Microscopy (FMM) are often used for their capabilities in detecting protein aggregates and degraded excipients such as polysorbates. Case studies and experimental results demonstrate how these tools provide high-throughput, low-volume analysis that enhances decision-making in formulation screening and manufacturing.
The Role of On-Demand Manufacturing and Derisking in Accelerating Early Clinical Trial Success
June 4th 2025Small pharmaceutical companies are constantly seeking innovative solutions to streamline early clinical trials. Adaptive clinical trials offer important benefits to sponsors and patients, both from a commercial and ethical standpoint. These trials offer flexibility and efficiency, especially in the early stages, where trial protocols can be adjusted based on interim data, such as introducing new doses or modifying participant sample size. However, adjusting manufacturing demand during an adaptive trial can be complicated, and strict regulatory requirements present significant challenges. On-demand manufacturing provides a robust solution, allowing for real-time supply and demand adjustments and improved trial flexibility. This paper explores how on-demand manufacturing meets the operational needs of adaptive trials and aligns with regulatory expectations.
Top 10 Cleanroom Problems That Can Be Prevented via Preventative Maintenance (May 2025)
May 16th 2025Cleanrooms require strict environmental control to maintain sterility, prevent contamination, and ensure seamless operations. Without a proactive preventative maintenance (PM) program, various issues can arise, leading to costly downtime, contamination risks, and operational inefficiencies. Below are ten common cleanroom problems that can be effectively mitigated through proper PM practices.
Optimizing cGMP Facility Design Space with a Hybrid Approach (May 2025)
May 16th 2025In this webcast, we review industry drivers and risks for capital construction, with a focus on cleanroom infrastructure, and discuss the goals of using a hybrid approach while demonstrating its application and benefits using real-world examples.
