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Analysis of Camelid Single-Domain Antibodies Using Agilent AdvanceBio SEC 120 Å 1.9 µm and AdvanceBio HIC Columns
Achieve high-resolution nanobody aggregate and fragment characterization with sub-2µm SEC UV and native LC/MS detection, and resolve important post translational modifications with non-denaturing HIC.
Mass Spectrometric Characterization of Antibody-siRNA Conjugates using the Agilent 6545XT AdvanceBio LC/Q-TOF
Denaturing (reversed-phase) and non-denaturing (size exclusion) analyses of novel siRNA conjugated monoclonal antibodies by LC/MS
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Elevate Your mAb Aggregate Analysis: High-resolution SEC with the Agilent 1290 Infinity II Bio LC System
How to optimize your ultra-high performance size exclusion separations, using biocompatible UHPLC instrumentation with attention to minimizing extra-column dead volume.
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An Improved Workflow for Profiling and Quantitation of Sialic Acids in Biotherapeutics
This work highlights a newly-released Agilent workflow for profiling and quantitation of sialic acids in biotherapeutic glycoproteins. This new workflow includes all the reagents necessary for sample preparation of sialic acids and also features a qualitative Sialic Acid Reference Panel ase well as quantitative NANA and NGNA standards.
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Analysis of a Synthetic Peptide and Its Impurities Using mass spectrometry compatible mobile phases with an Agilent AdvanceBio Peptide Plus column
The benefits of using a charged-surface modified C18 reversed-phase column for improved performance and peak shape of a synthetic peptide using LC/MS methodology.
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Seamless Method Transfer to the Agilent 1290 Infinity II Bio LC System Peptide-mapping analysis shows excellent performance and high method compatibility compared to the Agilent 1290 Infinity II LC System
Transitioning validated methods between instruments is sometimes a challenge. Using a peptide mapping example, we demonstrate exceptional data consistency between the Agilent 1290 Infinity II LC and the biocompatible Agilent 1290 Infinity II Bio LC.
How Shallow Can You Go? Refining charge variant analysis of mAbs with the Agilent 1290 Infinity II Bio LC System
Excellent rentention time and peak area reproducibility are possible even with highly challenging shallow gradients for ion exchange chromatography.
Improved Lifetime of Bio-Monolith Protein A Columns for Titer Determination
Learn how to increase column lifetime with an improved monolithic affinity chromatography column designed for rapid screening of monoclonal antibody titer in cell culture supernatant.
High Salt—High Reproducibility Analysis of antibody drug conjugates using hydrophobic interaction chromatography with the Agilent 1290 Infinity II Bio LC System
By removing iron from the LC flow path, the Agilent Infinity II Bio LC is perfectly suited for the high salt concentrations used in HIC-based drug-to-antibody ratio characterization.
Isolate and Reanalyze Pharma Impurities with the Agilent 1290 Infinity II Autoscale Preparative LC/MSD System
Learn about Agilent's Gly-X N-glycan sample preparation platform and available dye options for LC/FLD and LC/MS analysis of N-glycans from biotherapeutic glycoproteins.
Are Current Dissolved CO2 Measurement Technologies Good Enough?
November 15th 2024Download Part 2 of the DCO₂ White Paper Series and learn the shortcomings of current measurement technologies. As demonstrated in Part 1, Dissolved CO₂ is a Critical Process Parameter (CPP) in biopharma production processes. However, the sensors currently used to measure and monitor this CPP each have significant drawbacks.
Biopharma Downstream Critical Process Parameters (Nov 2024)
November 15th 2024Download our comprehensive white paper and gain valuable insights into the critical process parameters and advance sensor technologies that can revolutionize your Biopharma Downstream Process (DSP). Discover strategies and techniques to optimize efficiency, improve product quality, and ensure the safety and efficacy of your biopharmaceutical products.
White Paper: Measurement Challenges with Optical Dissolved Oxygen Sensors
November 15th 2024Are you missing sources of measurement error? Measurement of dissolved oxygen (DO) has long been known as a critical control parameter for optimizing cell growth in bioprocesses. Much has been written about different strategies for proper DO control. However, little information has been publicly available regarding optimization of the actual measurement accuracy and reducing common sources of process-related error. Our White Paper aims to divulge these details for the first time.
Cell Density Applications eBook
November 15th 2024Collect More Actionable Data and Optimize Yield with Real-Time Process Adjustments. Learn more how Viable Cell Density Monitoring works for your process in Hamilton’s eBook that features must-see examples of real applications using Hamilton viable cell density monitoring (Incyte) and total cell density monitoring (Dencytee).
